Ibidi were infected with in egfp carrying hiv 1nl4 3 6 7 105 μu of rt well pseudotyped with an r5 tropic envelope for 5 5 h or 48 h at 37 c respectively. A cell culture chamber for observation of the sample through the coverslip like bottom using high resolution microscopy. Hff spoc1 tet on cells were seeded into live cell imaging chamber slides 4 104 cells chamber μ slide 8 well.
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