Human Primary Microglia Cells

Human microglia primary cell culture frozen vial.

Human primary microglia cells.

Yet studying human microglia is challenging because of the rarity and difficulty in acquiring primary cells from human fetal or adult cns tissue. These primary cells go into senescence after the 2nd passage while the sv40 tranduced cells go beyond 30 passages. We describe a robust method for the derivation of human microglia from stem cells which are phenotypically and functionally comparable with primary microglia. Immortalized cells were controlled passaging side by side with the primary cells.

The hmc3 cell line was established through sv40 dependent immortalization of a human fetal brain derived primary microglia culture. The derivation of microglia from human stem cells provides systems for understanding microglial biology and enables functional studies of disease causing mutations. We are recently preparing the primary astrocytes and microglia cells. Comments resting hmc3 cells were strongly positive for the microglia macrophage marker iba1 positive for the endotoxin receptor cd14 but negative for the astrocyte marker gfap.

During maturation spherical monocytes adhere strongly to the culture surface displaying increasingly ramified morphology as they differentiate to microglia. Tardieu in 1995 through sv40 dependent immortalization of human microglial cells the detailed methodology for the preparation of primary cultures of human microglial cells was previously published briefly microglial cells were isolated by circular shaking from primary mixed cultures of human. Microglia are a major glial component of the central nervous system cns play a critical role as resident immunocompetent and phagocytic cells in the cns and serve as scavenger cells in the event of infection inflammation trauma ischemia and neurodegeneration in the cns. Primary neurons like all primary cells are isolated directly from human or animal nervous tissue unlike cell lines primary cells maintain the characteristics of their tissue of origin making them a biologically and physiologically relevant tool for the study of neuroscience.

The human microglial clone 3 cell line hmc3 was established in the laboratory of prof. Monday friday 8am 5pm pacific standard time. Immortalized human microglia im hm provided by innoprot have been developed by immortalizing primary human microglia with sv40 large t antigen. The human microglia cell line was constructed as the bv2 with a retrovirus that should have not infected mammalian cells.